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Image of agent fact sheet PDF: Francisella Tularensis (Tularemia)
Feature:
Agent Fact Sheet
Title:
Francisella Tularensis (Tularemia)
Publication:

UPMC Center for Health Security, 2014

Date posted:
February 26, 2014
Introduction:

F. tularensis is considered to be a serious potential bioterrorist threat because it is one of the most infectious pathogenic bacteria known-inhalation of as few as 10 organisms can cause disease-and it has substantial capacity to cause serious illness and death. The bacterium was developed into an aerosol biological weapon by several countries in the past.


Unless otherwise noted, all information presented in this article is derived from Dennis DT, Inglesby TV, Henderson DA, et al., for the Working Group on Civilian Biodefense. Tularemia as a biological weapon: medical and public health management. JAMA. 2001;285:2763-2773.

Background

Naturally occurring tularemia is a zoonotic disease caused by the bacterium Francisella tularensis, which is a hardy organism capable of surviving for weeks at low temperatures in water, moist soil, hay, straw, or decaying animal carcasses.

There are 4 subspecies of F. tularensis: F. tularensis subsp. tularensis (Type A), which is the most common type in North America, and is highly virulent in humans and animals. F. tularensis subsp. Holarctica (Type B), a less virulent type, responsible for human tularemia infection in Europe and Asia as well as North America; F. tularensis subsp. novocida, another less virulent type; and F. tularensis subsp. mediaasiatica, which is also of low virulence.

Small mammals such as voles, mice, squirrels, and rabbits are natural reservoirs for F. tularensis. These animals acquire tularemia through bites from ticks, fleas, and mosquitoes and also through contact with contaminated environments. Naturally acquired human infection can occur through bites from infected arthropods (usually ticks); contact with infected animal tissues or fluids; direct contact with or ingestion of contaminated water, food, or soil; or inhalation of aerosolized bacteria. Naturally acquired human infection tends to occur predominately in rural areas. F. tularensis is so infectious that simply opening a laboratory culture plate without adequate protective equipment can lead to infection.

      Microscopic image of F. tularensis
Microscopic image of F. tularensis. Source: National Institute of Allergy and Infectious Disease (NIAID) Laboratory of Intracellular Parasites, Tularemia Pathogenesis Section.

Tularemia as a Biological Weapon

F. tularensis is considered to be a serious potential bioterrorist threat because it is one of the most infectious pathogenic bacteria known-inhalation of as few as 10 organisms can cause disease-and it has substantial capacity to cause serious illness and death. The bacterium was developed into an aerosol biological weapon by several countries in the past.

Aerosol dissemination of F. tularensis in a populated area would be expected to result in the abrupt onset of large numbers of cases of acute, nonspecific respiratory febrile illness beginning 3 to 5 days later. (See "The History of Bioterrorism: Tularemia," a short video from the CDC.)

A World Health Organization (WHO) expert committee reported in 1970 that if 50 kg (110 pounds) of virulent F. tularensis were dispersed as an aerosol over a metropolitan area with a population of 5 million, there would be an estimated 250,000 incapacitating casualties, including 19,000 deaths.

Transmission

Human-to-human transmission is not known to occur.

Infection Control Measures

Since tularemia is not spread from person to person, it is not necessary to place patients diagnosed with tularemia in isolation.

Illness

Symptoms of tularemia depend on the virulence of the bacterial strain and route of infection. Symptoms of all forms of tularemia typically include fever, headache, body aches, and malaise. Symptoms usually develop within 3 to 5 days of infection; however, the incubation period can be 1 to 14 days. Naturally occurring tularemia infection can take several forms, as outlined in the table below. Pneumonic tularemia (the form expected from an aerosol release) is likely to cause typical symptoms of pneumonia (eg, fever, cough, and shortness of breath). Definitive diagnosis requires confirmation by laboratory testing.

Tularemia: Forms, Routes of Infection, and Symptoms
FormTypical route of infectionSymptoms
UlceroglandularHandling contaminated carcasses or a bite from an infected arthropod

Formation of an ulcer at the site of infection followed by swollen and painful regional lymph glands

GlandularHandling contaminated carcasses or a bite from an infected arthropod

Swollen and painful lymph glands without the development of ulcers

OculoglandularDirect contamination of the eye with F. tularensis

Pain, redness, swelling, and discharge of the eyes; development of an ulcer on the inside of the eyelid in some cases

OropharyngealEating or drinking contaminated food or water; inhaling aerosolized F. tularensis

Sore throat or tonsillitis; vomiting and diarrhea; possible swelling of the glands in the neck

PneumonicInhaling aerosolized F. tularensis; or secondary spread to lungs from another site of infection

Sore throat and swelling of the lymph nodes in the lungs; sudden fever, chills, headache, muscle aches, joint pain, dry cough, and progressive weakness

TyphoidalUnspecified

Systemic illness (fever, chills, headache, etc.) without indication of site of infection or localized symptoms

SepticUnspecified

Potentially severe and fatal; systemic illness (fever, chills, headache, etc.)

Patient typically appears toxic; may develop confusion and coma

Without prompt treatment, may lead to septic shock, acute respiratory distress syndrome, and organ failure

ulcer caused by tularemia infection
Ulcer caused by tularemia infection. Source: CDC Public Health Image Library (ID #2037).
leg lesion caused by tularemia
Leg ulcer caused by tularemia infection. Source: Office of Medical History, Office of the Surgeon General.

Clinicians who suspect tularemia should promptly obtain blood and other cultures, as appropriate, and alert the laboratory to the need for special diagnostic and safety procedures. F. tularensis may be identified through direct examination of secretions, exudates, or biopsy specimens using Gram stain, direct fluorescent antibody, or immunohistochemical stains. It can be grown from pharyngeal washings, sputum specimens, and even fasting gastric aspirates in a high proportion of patients with inhalational tularemia. It is only occasionally isolated from blood. Rapid diagnostic tests are not widely available; ancillary confirmatory testing via microscopic demonstration of F. tularensisusing fluorescent-labeled antibodies is a rapid diagnostic procedure performed in designated reference laboratories in the National Public Health Laboratory Network. Test results can be available within several hours if the laboratory is alerted and prepared. Growth of F. tularensis in culture is the definitive means of confirming the diagnosis and usually takes 24 to 48 hours in ideal conditions. However, in some instances, growth of the bacteria can be delayed up to 10 days.

The overall case fatality rate for untreated infections with Type A strains has been 5% to 15%, but in pneumonic or septic cases, without antibiotic treatment, the fatality rate has been as high as 30% to 60%. With treatment, the most recent fatality rates in the US have been 2%.

Prophylaxis and Treatment

Early antibiotic therapy is recommended for persons exposed to or infected with tularemia. Tetracyclines (eg, doxycycline), fluoroquinolones (eg, ciprofloxacin), and aminoglycosides (eg, streptomycin and gentamicin) are all effective treatments and doxycycline or a fluroquinolone can be used for prophylaxis after high risk exposure. Following a biological attack, treatment recommendations would depend on antibiotic susceptibility of the strain of bacteria used in the attack.

Since person-to-person transmission is not known to occur, post-exposure prophylaxis of close contacts with persons infected with tularemia is unnecessary.

Countermeasures

In the US, a live-attenuated vaccine derived from the avirulent live vaccine strain (LVS) developed by the Department of Defense (DoD) was used to protect laboratory personnel routinely working with F. tularensis and military high-risk personnel. The vaccine does not provide a high degree of protection from inhalational infection and is not currently in use.

See Also

(Last reviewed December 1, 2013)